Bioo Scientific has introduced AIR™ Barcoded Adapters which allow sample multiplexing in small RNA (ie. miRNA ) Next-Generation Sequencing reactions, while decreasing costs and increasing the number of samples that can be run simultaneously. miRNAs are small, single-stranded RNAs that regulate gene expression by partial complementary base pairing to specific mRNAs. Recently it has been shown that these small RNAs regulate gene expression by targeting mRNAs for translational repression and degradation. miRNAs modulate many cellular pathways including growth and proliferation, apoptosis and developmental timing. Multiplexed Next-Generation Sequencing using Bioo Scientific’s AIR Barcoded Adapters will significantly speed up the discovery and analysis of these important molecules.
AIR Barcoded Adapters, designed to work with Illumina Next-Generation Sequencers, provide flexibility in high-throughput sequencing applications. They significantly increase scale and throughput while reducing costs by allowing the user to pool multiple library preparations into a single sequencing reaction. The AIR Barcoded Adapters enable the user to detect rare sample events amongst hundreds of samples, across several time points and even multiple genomes. Predesigned AIR Barcoded Adapters, are available in sets of 10, 20, or 30 and were designed based on uniform melting temperature (Tm) and sequences that have unique color space. Once attached to a sample, they may be pooled together and sequenced in a single flow cell run. Analysis post run allows the user to identify the sequence barcode back to specific samples. AIR Barcoded Adapters can also be used to multiplex Next-Generation Sequencing reactions for piwi RNA (piRNA), small nucleolar RNA (snoRNA) and other small, noncoding RNAs.
According to Dr. Masoud Toloue, Senior Scientist at Bioo Scientific, “Sample barcoding technology maximizes Next-Generation Sequencing by allowing sample multiplexing, large library generation, cross-genomic and gene-drug treatment studies. This tagged technology improves sequencing experimental design to such a magnitude; it is likely all future Next-Generation Sequencing runs will be performed with barcodes.”
About Bioo Scientific Corporation
Bioo Scientific Corporation is an Austin, TX based biotechnology company that provides innovative solutions to the life science industry. Bioo Scientific offers a complete portfolio of products and services for academic, pharmaceutical, biotechnology and molecular diagnostics laboratories, and for organizations that perform food and feed safety analysis.
Bioo Scientific’s products are used around the world and can be purchased both directly or through our global distribution network that markets our products in more than 40 countries. For more information about Bioo Scientific, visit www.biooscientific.com.
Monday, January 25, 2010
Thursday, December 10, 2009
Targeted In Vivo siRNA Delivery
Bioo Scientific has introduced the patent pending T3™ Conjugation Kit for targeted in vivo delivery of siRNA or any other RNAi agent. The T3 Conjugation Kit can also be used for targeted in vitro delivery of siRNA in primary cells or cell lines, which have mixed cell populations. Bioo Scientific has built upon its expertise in RNAi delivery to create a technology that will offer customers the ability to effectively delivery siRNA or microRNA (miRNA) into any targeted cell or tissue type they are studying.
RNAi has revolutionized biology by allowing researchers to directly observe the effects of the loss of function of specific genes in mammalian systems. However, achieving efficient in vivo delivery of siRNA to the appropriate target cell has been a major hurdle in the advancement of using RNAi in gene function studies and as a therapeutic agent. This hurdle can now be easily overcome using the T3 Conjugation Kit.
The T3 technology functions by conjugating a T3 Carrier to an antibody that is specific to a receptor or other extracellular domain specific to the targeted cell or tissue type. The T3 Carrier-Antibody Conjugate can then be loaded with an RNAi agent such as siRNA or miRNA. The RNAi agent loaded T3 Conjugate may then be administered systemically or locally to an animal (or cultured cells) where it binds to and is internalized by cells recognized by the antibody. Once inside the cell, the RNAi agent is designed for release from T3 allowing silencing of its intended target. The T3 Conjugation Kit technology will propel the validation of in vivo work, leading to a better understanding of cellular pathways, the identification of novel drug targets, and the ability to more efficiently deliver RNAi agents as drugs.
According to Dr. Masoud Toloue, Senior Scientist at Bioo Scientific, “siRNA/miRNA delivery remains the largest hurdle for in vivo research application and all efforts to make RNAi a real therapeutic modality. With the launch of our T3 Conjugation Kit, both scientists and clinicians will have access to an antibody based siRNA/miRNA delivery vehicle that allows the user to choose any antibody and siRNA/miRNA combination for their research. For the first time, T3’s targeting technology enables users to concentrate RNAi mediator on their tissue/organ or tumor of focus while limiting systemic toxicity and significantly reducing current dose requirements. As the first commercial siRNA targeted delivery vehicle to market, T3 will advance and revolutionize the entire siRNA delivery arena.” This research was funded in part by grants from the National Science Foundation.
About Bioo Scientific Corporation
Bioo Scientific Corporation is an Austin, TX based biotechnology company that provides innovative solutions to the life science industry. Bioo Scientific offers a complete portfolio of products and services for academic, pharmaceutical, biotechnology and molecular diagnostics laboratories, and for organizations that perform food and feed safety analysis.
RNAi has revolutionized biology by allowing researchers to directly observe the effects of the loss of function of specific genes in mammalian systems. However, achieving efficient in vivo delivery of siRNA to the appropriate target cell has been a major hurdle in the advancement of using RNAi in gene function studies and as a therapeutic agent. This hurdle can now be easily overcome using the T3 Conjugation Kit.
The T3 technology functions by conjugating a T3 Carrier to an antibody that is specific to a receptor or other extracellular domain specific to the targeted cell or tissue type. The T3 Carrier-Antibody Conjugate can then be loaded with an RNAi agent such as siRNA or miRNA. The RNAi agent loaded T3 Conjugate may then be administered systemically or locally to an animal (or cultured cells) where it binds to and is internalized by cells recognized by the antibody. Once inside the cell, the RNAi agent is designed for release from T3 allowing silencing of its intended target. The T3 Conjugation Kit technology will propel the validation of in vivo work, leading to a better understanding of cellular pathways, the identification of novel drug targets, and the ability to more efficiently deliver RNAi agents as drugs.
According to Dr. Masoud Toloue, Senior Scientist at Bioo Scientific, “siRNA/miRNA delivery remains the largest hurdle for in vivo research application and all efforts to make RNAi a real therapeutic modality. With the launch of our T3 Conjugation Kit, both scientists and clinicians will have access to an antibody based siRNA/miRNA delivery vehicle that allows the user to choose any antibody and siRNA/miRNA combination for their research. For the first time, T3’s targeting technology enables users to concentrate RNAi mediator on their tissue/organ or tumor of focus while limiting systemic toxicity and significantly reducing current dose requirements. As the first commercial siRNA targeted delivery vehicle to market, T3 will advance and revolutionize the entire siRNA delivery arena.” This research was funded in part by grants from the National Science Foundation.
About Bioo Scientific Corporation
Bioo Scientific Corporation is an Austin, TX based biotechnology company that provides innovative solutions to the life science industry. Bioo Scientific offers a complete portfolio of products and services for academic, pharmaceutical, biotechnology and molecular diagnostics laboratories, and for organizations that perform food and feed safety analysis.
Wednesday, September 30, 2009
Bioo Scientific Offers Rapid Tests to Detect E. coli and Salmonella Contamination in Food
Bioo Scientific, a world leader in food safety testing, today announced the availability of its MaxSignal® E. coli O157:H7 and Salmonella Test Strip Kits, lateral flow test kits developed to simplify the analysis of pathogen contamination in food. The MaxSignal® E. coli O157:H7 and Salmonella Test Strip Kits are easy to use, accurate and affordable pathogen detection systems with sensitivity equal to or greater than that found in ELISAs. These kits do not require specialized training or skills to use.
According to Lance Ford, VP of Research and Business Development for Bioo Scientific, “Bioo Scientific continues to expand its portfolio of innovative products, which allow customers to ensure that their food and feed products meet global safety standards. The MaxSignal® E. coli O157:H7 and Salmonella Test Strip Kits broaden the range of choices in pathogen testing we offer to food companies.”
About Bioo Scientific Corporation
Bioo Scientific Corporation is an Austin, TX based biotechnology company that provides innovative solutions to the life science industry. Bioo Scientific offers a complete portfolio of products and services for academic, pharmaceutical, biotechnology and molecular diagnostics laboratories, and for organizations that perform food and feed safety analysis.
As a world leader in food safety testing, Bioo Scientific is dedicated to improving the safety of the world’s food and feed supply by developing accurate, affordable tools, which allow companies to readily screen for pathogens and contaminants. Bioo Scientific’s products are used around the world and can be purchased both directly or through our global distribution network that markets our products in more than 40 countries. For more information about Bioo Scientific, visit www.biooscientific.com.
According to Lance Ford, VP of Research and Business Development for Bioo Scientific, “Bioo Scientific continues to expand its portfolio of innovative products, which allow customers to ensure that their food and feed products meet global safety standards. The MaxSignal® E. coli O157:H7 and Salmonella Test Strip Kits broaden the range of choices in pathogen testing we offer to food companies.”
About Bioo Scientific Corporation
Bioo Scientific Corporation is an Austin, TX based biotechnology company that provides innovative solutions to the life science industry. Bioo Scientific offers a complete portfolio of products and services for academic, pharmaceutical, biotechnology and molecular diagnostics laboratories, and for organizations that perform food and feed safety analysis.
As a world leader in food safety testing, Bioo Scientific is dedicated to improving the safety of the world’s food and feed supply by developing accurate, affordable tools, which allow companies to readily screen for pathogens and contaminants. Bioo Scientific’s products are used around the world and can be purchased both directly or through our global distribution network that markets our products in more than 40 countries. For more information about Bioo Scientific, visit www.biooscientific.com.
Tuesday, July 14, 2009
Sensitive Detection of Melamine Contamination
Today BIOO Scientific announced the expansion of its portfolio of food safety testing solutions with a new kit which improves the detection of melamine in food. Melamine contamination has recently been identified in infant formula and other milk-based products from China. Melamine is a dangerous, nitrogen rich organic compound that is illegally added to food and feed to increase the apparent protein content values of food products. Consumption of melamine causes highly insoluble melamine cyanurate crystals to form in the kidney, which damage renal cells and can result in renal failure or death. The recent establishment of a 1µg/g (1ppm) safety threshold for melamine in infant foods has led to an immediate need for sensitive melamine detection methods.
Bioo Scientific’s MaxSignal Melamine Enzymatic Assay Kit has been developed to meet the industry’s need for increased sensitivity. The MaxSignal Melamine Enzymatic Assay Kit is a rapid, inexpensive aqueous extraction method that is able to detect down to 0.2 ppm of melamine. According to Dr Joe Krebs, Director of Protein Chemistry and Engineering at Bioo Scientific, “The MaxSignal Melamine Enzymatic Assay Kit does not require expensive and time-consuming mass spectrometry and immunoassay techniques characteristic of currently available methods. Our novel enzyme-based method offers food safety analysts a new choice for sensitive and cost-effective melamine detection in food samples.”
Bioo Scientific’s MaxSignal Melamine Enzymatic Assay Kit has been developed to meet the industry’s need for increased sensitivity. The MaxSignal Melamine Enzymatic Assay Kit is a rapid, inexpensive aqueous extraction method that is able to detect down to 0.2 ppm of melamine. According to Dr Joe Krebs, Director of Protein Chemistry and Engineering at Bioo Scientific, “The MaxSignal Melamine Enzymatic Assay Kit does not require expensive and time-consuming mass spectrometry and immunoassay techniques characteristic of currently available methods. Our novel enzyme-based method offers food safety analysts a new choice for sensitive and cost-effective melamine detection in food samples.”
Tuesday, June 2, 2009
Bioo Scientific’s Toxicity Assay Kits Increase the Efficiency of Drug Development
Since toxicity is one of the leading causes of drug failures, measuring preclinical toxicity is a critical drug development function. Accurate in vivo toxicity assays are essential to lowering attrition and increasing efficiency in drug development.
Bioo Scientific announced today the launch of their MaxDiscovery™ Toxicity Assay Kits. These kits monitor in vivo toxicity by measuring liver and heart function in serum samples. They provide simple, convenient, colorimetric determination of well-characterized functional markers of for liver and heart. They are ideal tools to monitor toxic effects caused by administration of siRNA, DNA plasmids, transfection agents or other therapeutic treatments during preclinical studies. The assays can be used in a range of mammals, including mice and rats, providing a powerful tool to assess and reduce the toxicity of drugs formulations and to improve drug delivery methods.
According to Dr Joe Krebs, Director of Protein Chemistry and Engineering at Bioo Scientific, “MaxDiscovery™ Toxicity kits are designed to provide researchers with rapid, cost-effective tools required to address the escalating challenges of drug development in today's competitive landscape.”
Assays are available for alanine transaminase, aspartate transminase, alkaline phosphatase, lactate dehydrogenase, cholesterol, and creatine kinase.
About Bioo Scientific
Bioo Scientific Corporation is a global biotechnology tools company, headquartered in Austin, Texas. Bioo Scientific provides innovative products and services to academic researchers, pharmaceutical and biotechnology companies, molecular diagnostics laboratories, and customers who perform food and feed safety analysis.
Bioo Scientific announced today the launch of their MaxDiscovery™ Toxicity Assay Kits. These kits monitor in vivo toxicity by measuring liver and heart function in serum samples. They provide simple, convenient, colorimetric determination of well-characterized functional markers of for liver and heart. They are ideal tools to monitor toxic effects caused by administration of siRNA, DNA plasmids, transfection agents or other therapeutic treatments during preclinical studies. The assays can be used in a range of mammals, including mice and rats, providing a powerful tool to assess and reduce the toxicity of drugs formulations and to improve drug delivery methods.
According to Dr Joe Krebs, Director of Protein Chemistry and Engineering at Bioo Scientific, “MaxDiscovery™ Toxicity kits are designed to provide researchers with rapid, cost-effective tools required to address the escalating challenges of drug development in today's competitive landscape.”
Assays are available for alanine transaminase, aspartate transminase, alkaline phosphatase, lactate dehydrogenase, cholesterol, and creatine kinase.
About Bioo Scientific
Bioo Scientific Corporation is a global biotechnology tools company, headquartered in Austin, Texas. Bioo Scientific provides innovative products and services to academic researchers, pharmaceutical and biotechnology companies, molecular diagnostics laboratories, and customers who perform food and feed safety analysis.
Monday, April 27, 2009
Efficient In Vivo siRNA Delivery
MaxSuppressor™ In Vivo RNA-LANCEr™ II is an exciting new product from Bioo Scientific that simplifies in vivo RNAi experiments. The RNA-LANCEr II is a non-viral transfection agent optimized for the efficient in vivo introduction of siRNA, miRNA, and other RNAi agents directly into tissues. It is easy to use – simply mix with your RNAi agent, extrude or sonicate the mixture, and inject it into your animal. MaxSuppressor In Vivo RNA-LANCEr II offers consistent, reliable silencing using minimal amounts of RNAi agents. To learn more about how Bioo Scientific's MaxSuppressor In Vivo RNA-LANCEr II can simplify you in vivo RNAi experiments visit Bioo Scientific.
Monday, December 22, 2008
The DNA Assembler - Rapidly Construct Biochemical Pathways
Researchers at the University of Illinois at Urbana-Champaign have created the DNA Assembler, a novel way to rapidly construct biochemical pathways in yeast. The DNA Assembler incorporates homologous recombination to allow scientists to assemble entire biochemical pathways in a single step only requiring a simple DNA prep and a one-step yeast transformation. Advantages of the DNA assembler method include:
- Assembly of multiple-gene biochemical pathways in a single step.
- Most rapid and efficient approach to construct large recombinant DNA molecules.
It can be used to construct custom-designed DNA molecules not only on a plasmid but also on a chromosome with the potential to assemble a very large biochemical pathway or even a whole genome.The DNA Assembler represents a powerful tool in the construction of biochemical pathways for synthetic biology, metabolic engineering and functional genomics studies. To learn more about it read the original paper published in the Dec 12th issue of Nucleic Acids Research,http://nar.oxfordjournals.org/cgi/content/short/gkn991v1?rss=1
- Assembly of multiple-gene biochemical pathways in a single step.
- Most rapid and efficient approach to construct large recombinant DNA molecules.
It can be used to construct custom-designed DNA molecules not only on a plasmid but also on a chromosome with the potential to assemble a very large biochemical pathway or even a whole genome.The DNA Assembler represents a powerful tool in the construction of biochemical pathways for synthetic biology, metabolic engineering and functional genomics studies. To learn more about it read the original paper published in the Dec 12th issue of Nucleic Acids Research,http://nar.oxfordjournals.org/cgi/content/short/gkn991v1?rss=1
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