Shotgun protein sequencing

What is shotgun protein sequencing? It is a new, quicker way to sequence monoclonal antibodies that was developed by bioinformatics researchers at the University of California, San Diego and Genentech. It is a new protein sequencing method that reduces the time required to sequence an unknown antibody to under 36 hours. Currently, it takes weeks to months to sequence an antibody.

This technique is described in detail in the article “Automated de novo protein sequencing of monoclonal antibodies” published in the December 2008 issue of Nature Biotechnology.